HER2/neu Expression in Gastric and Esophagogastric Junction Adenocarcinoma

نویسندگان

  • N Sunitha
  • G Champaka
  • Rekha V Kumar
  • K C Lakshmaiah
چکیده

Background: Gastric cancer is one of the most commonly diagnosed cancer and one of the most common causes of cancerrelated deaths worldwide. HER-2/neu, a well-recognized factor, is indicated in the development of certain human solid tumors, notably breast cancer. High expression of HER2/neu has correlated with poor prognosis. Studies reveal combination of treatment with antibody targeting HER-2/neu protein (Trastuzumab) and chemotherapy significantly improves overall survival in patients with gastric and esophagogastric junctional (EGJ) adenocarcinomas with HER-2/neu protein overexpression. We aimed to investigate the frequency of Her2/neu expression and association with clinicopathological parameters in our gastric and EGJ adenocarcinoma series to make a contribution to the emerging data on this oncogene. Materials and Methods: A total of 100 cases, including biopsy and resection specimens between November 2012 and December 2014, were selected. Her2/neu expression was determined by immunohistochemistry (IHC) on tumor tissues. Samples with IHC 2+ scores/equivocal cases were tested using fluorescent in situ hybridization (FISH). Result: We found 8% positivity for HER2/neu protein expression (IHC 3+ and IHC 2+/FISH +). Gene amplification in 5 cases revealed 100% concordance with IHC 3+. The findings of our study are in concordance with international data. We also observed a relationship of intestinal phenotype, EGJ tumors, and well-differentiated adenocarcinoma with HER2 overexpression. Conclusion: The findings of our study suggest a relationship of EGJ tumors, intestinal phenotype, and well-differentiated adenocarcinoma with HER2 overexpression.

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تاریخ انتشار 2017